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Table 2 Melanin and L-tyrosine production in shake flask cultures with mineral medium supplemented with 10 g/L glucose*

From: Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose

Strain

Melanin titer

L-TYR

L-TYR max1

L-Tyr/melanin conversion

(mg/L)

(mg/L)

(mg/L)

(%)

W3110M

0

0

0

0

W3110MG

15.1 ± 3.7

7.10 ± 4.5

6.9 ± 6.4

198

W3110MGT

39.9 ± 2.6

27.5 ± 12.1

55.1 ± 14.9

66

VH33MGT

72.4 ± 1.5

70.6 ± 13.9

91.0 ± 2.5

72

VH33MGTR

155 ± 34.8

263 ± 38.9

456 ± 29.4

30

VH33MGTRK

112 ± 14.8

66.4 ± 0.93

158 ± 46.3

64

  1. *CuSO4 was added to culture medium 16 h after inoculation.
  2. 1Cultures were performed in medium lacking CuSO4.