Table 1 E. coli strains and plasmids used in this study
From: Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose
Strain or plasmid | Relevant features | Reference or source |
|---|---|---|
W3110 | F-, λ-, INV (rnnD-rnnE)1 | ATCC27325 |
VH33 | W3110 ΔptsH, ptsI, crr::km, ΔlacI, lacZ::lox P, Ptrc-galP | [20] |
VH33tyrR | VH33 ΔtyrR::FRT | [18] |
W3110M | W3110/pACMutmelA I | This work |
W3110MG | W3110/pRW300, pACMutmelA I | This work |
W3110MGT | W3110/pRW300, pMmelAtyrCpheA CM | This work |
VH33MGT | VH33/ pRW300, pMmelAtyrCpheA CM | This work |
VH33GTR | W3110tyrR/pRW300, pACYCtyrCpheA CM | This work |
VH33MGTR | VH33tyrR/pRW300, pMmelAtyrCpheA CM | This work |
VH33MGTRK | VH33tyrR/pRW300, pACYCtyrCpheA CM, pCLtktA | This work |
Plasmids | Relevant features | Reference or source |
pTrc99A | Expression plasmid carrying the trc promoter, a multiple cloning site, the T1 and T2 rrnB terminator sequences, the bla and lacIq genes. | [21] |
pACYC184 | Cloning plasmid carrying genes cat and tet. p15A replication origin. | [22] |
pRW300 | aroGfbr under control of the lacUV5 promoter carries lacIq and tet genes. Replication origin from pBR322. | [16] |
pCLtktA | Carried genes tktA and aada. Derivative of plasmid pCL920 with pSC101 origin of replication. | [16] |
pTrcMutmelA | Gene MutmelA cloned in pTrc99A. | [9] |
pTrctyrCpheA CM | tyrC and pheA CM under the control of trc promoter. pheA CM codes for the chorismate mutase domain of P-protein. | [10] |
pACMutmelA I | Gen MutmelA cloned in pACY184. | This work |
pMutmelAtyrCpheA CM | Genes tyrC and pheA CM cloned in pACMutmelAI. | This work |
pACYCtyrCpheA CM | Genes tyrC and pheA CM cloned in pACYC184. | This work |