Table 1 Strains and plasmids used in this study
Strain | Description | Reference |
|---|---|---|
R. eutropha H16 | R. eutropha wildtype strain currently classified as Cuparividus necator | ATCC 17669 |
R. eutropha H16 ∆2303 | H16 Δ(H16_A0459-0464, H16_A1526-1531); Δbeta ox; mutant is deficient in native-oxidation | [11] |
E. coli DH10B | F' proA+B+lacIq ∆lacZM15/ fhuA2 ∆(lac-proAB) glnV galR (zgb-210::Tn10) TetSendA1 thi-1 ∆(hsdS-mcrB)5 | NEB |
E. coli S17 | E. coli host strain for transconjugation | [12] |
Plasmid | Description | Reference |
pCM62 | Broad host-range plasmid IncP group; ampR, tetR | [8] |
pBBR1MCS | Broad host-range plasmid compatible with IncQ, IncP, IncW, and colE1; kanR | [9] |
pKT230 | Broad host-range plasmid IncQ group; kanR | [10] |
pBADrfp | pBBR1MCS derivative; PBAD_rfp | [13] |
pBbE8c-RFP | colE1; PBAD_rfp; cmR | [14] |
pBbA8a-RFP | p15a; PBAD_rfp; ampR | [14] |
pKTrfp | pKT230 derivative; PBAD_rfp; cmR | This study |
pCMrfp | pCM62 derivative; PBAD_rfp; ampR, tetR | This study |
pCM271rfp | pCMrfp with TrfA R271C mutation | This study |
pCM273rfp | pCMrfp with TrfA R273C mutation | This study |
pCM291rfp | pCMrfp with TrfA Q291G mutation | This study |
pBADTrfp | pBADrfp derivative; PBAD_T7 stem-loop_rfp | This study |
pXylsTrfp | pBADTrfp derivative; Pxyls/PM_T7 stem-loop_rfp | This study |
pUV5Trfp | pBADTrfp derivative; PlacUV5_T7 stem-loop_rfp | This study |
pIUV5Trfp | pBADTrfp derivative; lacIq; PlacUV5_T7 stem-loop_rfp | This study |
pTetTrfp | pBADTrfp derivative; Ptet_T7 stem-loop_rfp | This study |
pProErfp | pBADTrfp derivative; PproE_ rfp | This study |
pProSrfp | pBADTrfp derivative; PproS_ rfp | This study |
pBADT7Trfp | pBADTrfp derivative; PBAD_T7 polymerase; PT7_T7 stem-loop_rfp | This study |
pYIUV5Trfp | pBADTrfp derivative; lacY lacIq; PlacUV5_T7 stem-loop_rfp | This study |
pBADTcalRBSrfp | pBADTrfp derivative; PBAD_T7 stem-loop_calRBSrfp_rfp | This study |
pBADTnrdDRBSrfp | pBADTrfp derivative; PBAD_T7 stem-loop_nrdDRBS_rfp | This study |
pKTTrfp | pKT230 derivative; PBAD_ T7 stem-loop_rfp; cmR | This study |
pCMTrfp | pCM62 derivative; PBAD_ T7 stem-loop_rfp | This study |
pCM271Trfp | pCM62 derivative; pCMTrfp with TrfA R271C mutation | This study |
pCM271TcalRBSrfp | pCM271rfp derivative; PBAD_T7 stem-loop_calRBSrfp_rfp | This study |
pBADTHC | pBADrfpT derivative; PBAD_T7 stem-loop_aar _adc | This study |
pKTTHC | pKTrfp derivative; PBAD_T7 stem-loop_aar _adc | This study |
pCMTHC | pCM62 derivative; PBAD_T7 stem-loop_aar _adc | This study |
pCM271THC | pCM271rfp derivative; PBAD_T7 stem-loop_aar _adc | This study |
pBADHC | pBADrfp derivative; PBAD_aar _adc | This study |
pXylsTHC | pBADrfp derivative; Pxyls/PM_T7 stem-loop_aar _adc | This study |
pYIUV5THC | pYIUV5Trfp derivative; PlacUV5_T7 stem-loop_aar _adc | This study |
pBADTcalRBSHC | pBADTHC derivative; PBAD_T7 stem-loop_calRBSaar_aar _calRBSadc_adc | This study |
pBADTnrdDRBSHC | pBADTHC derivative; PBAD_T7 stem-loop_nrdDRBS_aar _ nrdDRBS_adc | This study |
pCM271TcalRBSHC | pCM271rfp derivative; PBAD_T7 stem-loop_ calRBSaar_aar _calRBSadc_adc | This study |