Figure 5From: Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgarisColony PCR analysis of the 18S rRNA from other species of Chlorella . Cells were disrupted in either 10 mM TE (A) or 0.2% SDS (B) and followed by PCI extraction for lipid removal. PCI denotes Phenol:Chloroform:Isoamyl Alcohol (Cat# P2069, Sigma).Back to article page