Figure 6

Overexpression of a predicted R. opacus PD630 GapA homolog results in a decrease in TAG accumulation. A. We hypothesize that GapA and GapN compete for G3P during glycolysis in R. opacus PD630. B. Extrachromosomal expression of GapA results in a decrease in TAG accumulation. Lipid extracts from wildtype R. opacus PD630 expressing either GFP, TadD or the R. jostii RHA1 GapA homolog encoded by the ro07177 gene grown in minimal medium supplemented with gentamycin were resolved using TLC. As described above, expression of the TadD protein resulted in an increase in TAGs while expression of the ro07177 protein resulted in a dramatic decrease in TAGs as compared to the wildtype strain containing the empty vector pDPM70.