Engineering NAD+ availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production

Table 3 Primers used in this study ^a

Primer	Sequence(5′-3′)	Function
gldA-F0	TGCTGTATATAGCGCCGCACAAG	gldA _cloning
gldA-R0	AGGTTGGTATTGGCCTGGATTTG
gldA-F1	CAATTTCACACAGGAAACAGACCATGGACCGCATTATTCAATCAC	gldA _amplification
gldA-R1	GTGTATATCTCCTTC TCTAGTAGCGATCTATTATTCCCACTCTTGCAGG
Nox-F1	CTACTAGAGAAGGAGA TATACACATGAAAGTCGTAGTCGTAGG	nox _amplification
Nox-R1	CAAAACAGCCAAGCTTGCATGCCTGCAGTTACATATTTTCTAAAGCGGCTTG
gapAP1-F1	TCGGATATC GAGGCGAGTCAGTCGCGTAATGC	Promoter gapAP1 amplification
gapAP1-R1	ACCGAATTC GATCTCATATGTTCCA CCAGCTATTTGTTAG
gntT105P-F1	CCGTTGATATC TGAAAGGTGTGCGCGATCTCAC	PromotergntT105P amplification
gntT105P-R1	GGAATTC TATCTCCT TATTCATTTGCGCTGGGTAACGTCAATTT
ntt4-F1	TTCGAGCTC ATGAGTAAAACAAACCAGG
ntt4-R1	AGAGGATCC TTAGTGATGATGATGATGATGTTTTTTTATAAAAG	ntt4 amplification
gntT105P-F2	CAAACTCTTTTTGTTTATTTTTCTAAATACATGAAAGGTGTGCGCGATCTC	gntT105P + ntt4 amplification
ntt4-R2	CGTTTTATTTGATGCCTGGATCCGCGTCGACTCTAGAGGATCC
T-F1	GGATCCTCTAGAGTCGACGCGGATCCAGGCATCAAATAAAACG	Terminator BBa_B0015 amplification
T-R1	GTATTTAGAAAAATAAACATATAAACGCAGAAAGGCCCAC

^aThe restriction sites were bolded and the ribosome binding sites (RBS) were underlined.

ISSN: 1475-2859