Figure 4

DHA production by E. coli YJE005 and YJE006. Cells were cultivated at 37°C for 8 h after being induced by IPTG, and collected for biotransformation experiments with 20 g/L glycerol and 0.2 mM NAD⁺ in 5 mL of reaction buffer in 50-mL test tubes at 37°C, 200 rpm for 10 h. (A) DHA specific titer; (B) Initial and end-point enzymatic activity of GldA and NOX; (C) Initial and end-point cellular NAD(H) level. All data represent the averages ± SDs for three independent clones.