Figure 7

Fed-batch bioreactor cultivation of NCYC3825 (A.B.) and A18 (C.D.) strains in a glycerol-based media. Representative kinetics of glycerol utilization (g L^-1) and citric acid production (g L^-1) (A.C.), biomass formation (DCW, g L^-1) and percentage of total lipid fraction in DCW (% DCW) (B.D.) of the recombinant Y. lipolytica NCYC3825 strain (A.B.) and the parental strain A18 (C.D.) during bioreactor fed-batch cultivation in a glycerol-based medium. Experimental conditions are described in “Materials and methods - Microbial strains, media and culture conditions” section, and quantitative summary is provided in (Table 1).