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Figure 1 | Microbial Cell Factories

Figure 1

From: Recombinant polypeptide production in E. coli: towards a rational approach to improve the yields of functional proteins

Figure 1

Expression rate controls the protein native folding in the cytoplasm. The recombinant polypeptide synthetized by the ribosomal machinery is mostly dependent on one/more chaperones and foldases to reach its native conformation. Disequilibrium between expression rate and folding capacity (a) will result in the accumulation of aggregates. This fate can be prevented by both reducing the expression rate of the recombinant protein and favoring the accumulation of chaperones and foldases (b).

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Microbial Cell Factories

ISSN: 1475-2859

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