Follow-up of parallelized cultures of E. coli pfis::gfpAAV in 200 mL mini-bioreactors. The three different cultures have been carried out in batch mode at three temperatures, 30°C, 37°C and 42°C, and monitored by automated flow cytometry. A: Evolution of optical density at 600 nm. B: Recording of dissolved oxygen profiles. C: Monitoring of GFP signal under the control of the fis promoter and D: PI uptake, by automated flow cytometry. Cultures were performed in duplicate.