Evolution of membrane permeability according to PI uptake during A the chemostat phase and B the chemostat phase with additional glucose pulses. All the results are displayed on a FL3/FL1 dotplot on the basis of the analysis of 40,000 microbial cells by automated flow cytometry. The FL3 channel corresponds to the red fluorescence emitted after PI uptake and the FL1 channels corresponds to the green fluorescence emitted by the GFP synthesized by cells. Representative results from two independent cultivations. Legend: R1: healthy cells; R2: intermediate cells; R3: damaged cells.