The PUB1 deletion increases glycerol production and extends CLS during winemaking. A) Natural grape juice fermentations of the PUB1 deletion strain and its parental strain L2056. Cell viability over time was measured as cfu/mL and fermentation evolution was followed by measuring sugar consumption. Ethanol (B), acetic acid (C) and glycerol (D) production of the fermentation described in Panel A. Experiments were done in triplicate and the mean and standard deviation are provided. **p<0.01, unpaired t-test, two-tailed.