Sirtuin gene overexpression modulates life span under winemaking conditions. A) Western blot showing Sir2 levels on days 1 and 5 of natural grape juice fermentation. Wild-type commercial strain L2056 and its derivatives with SIR2 under the control of promoters SPI1 and MET17 are included. B) Natural grape juice fermentations of the SIR2-overexpressing strains described in Panel A. Cell viability over time was measured as cfu/mL and fermentation evolution was followed by measuring sugar consumption. C) Survival curve of the SIR2-overexpressing strains under winemaking conditions. Cell viability on day 8 of Panel B was taken as 100% survival. The survival curves of the HST3- (D) and GCN5- (E) overexpressing strains. Other conditions as in Panel C. Experiments were done in triplicate and the mean and standard deviation are provided.