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Table 1 Kinetic parameters K m and V max of glucose uptake in yeast cells harvested during the three fermentation curves examined in this study (Figure 1 ) corresponding to different nitrogen supplementations of the fermentation medium: complete fermentation (CF), nitrogen-limited fermentation (LF) and refed fermentation (RF)

From: Impact of assimilable nitrogen availability in glucose uptake kinetics in Saccharomyces cerevisiae during alcoholic fermentation

Incubation Time (h)

Km(mM)

Vmax(μmol h-110-8cells)

 

CF

LF

RF

CF

LF

RF

6

99.2 ± 6.5

108.5 ± 10.4

 

23.2 ± 0.7

21.9 ± 1.2

 

24

36.6 ± 3.5

81.7 ± 9.4

 

13.9 ± 0.4

11.5 ± 0.6

 

48

34.2 ± 4.4

43.8 ± 5.6

 

11.0 ± 0.5

4.7 ± 0.2

 

72

ND

31.5 ± 2.9

AS

ND

4.8 ± 0.1

AS

80

67.5 ± 14.9

38.9 ± 6.6

33.2 ± 3.3

9.1 ± 0.8

5.1 ± 0.6

12.9 ± 0.4

 

2.1 ± 0.6

  

3.5 ± 0.1

  

96

45.2 ± 10.2

39.2 ± 8.6

58.2 ± 9.1

8.6 ± 0.7

4.9 ± 0.8

8.7 ± 0.8

 

1.9 ± 0.3

 

2.7 ± 0.4

3.8 ± 0.2

 

1.0 ± 0.1

144

ND

ND

33.1 ± 3.5

ND

ND

7.3 ± 0.4

   

1.3 ± 0.3

  

1.3 ± 0.2

  1. ND: Not determined.
  2. AS: Ammonium supplementation.