Table 2 Oligonucleotides used in this study
Name | Sequence (5’ to 3’) |
|---|---|
(1) pre-pBR322-ori-EcoRV | CGGATATCTGAGCAAAAGGCCAGCAAAA |
(2) pre-blaP-SwaI-EcoRV | CGGATATCCAATTTAAATGCGGAACCCCTATTTGTTTATT |
(3) 5_EcoRV_5_ssu | TTGGATATCTTGGCGAAACGCC |
(4) EcoRV_3_ssu | GATTTAGATATCGGTGAACTTTCGGG |
(5) Pci_linker_sat4 | GCTCTAGAGACATGTCGCCAGCGTCGACCG |
(6) Pci_linker_sat5 | GCTCTAGAGACATGTTGACCGACACGGCCGCTG |
(7) BglII_linker_sat6 | GAAGATCTGCCATGACCTCGAGGCTCGTC |
(8) LEXSY_A264 | CATCTATAGAGAAGTACACGTAAAAG |
(9) NcoI_linker_sat6 | GCTCTAGAGCCATGGTGACCGACACGGCCGCTG |
(10) NcoI_linker_sat7 | GCTCTAGAGCCATGGCGACCGACACGGCCGCTG |
(11) LMB3 | CAG GAA ACA GCT ATG AC |
(12) LEXSY_scFv_KpnI | GGTGGGTACCCCGTTTGATTTCCACCTTGGTC |