Table 1 Vectors and constructs used in this study
Name | Characteristics | Reference |
|---|---|---|
pLEXSY-sat2 | Expression vector pLEXSY-sat2 with sat gene (streptothricine acetyltransferase) allowing selection of recombinant LEXSY strains with antibiotic NTC; and Amp for DH10B | Jena Bioscience (Cat.-No. EGE-234) |
pLTEX-1 | Based on pLEXSY-sat2; with reduced pBlueScript backbone and changed RE-site for vector linearization prior transfection (SwaI to EcoRV) | this study |
pLTEX-2 | based on pLTEX-1; changed SP sequence | this study |
pLTEX-3 | based on pLTEX-1; changed SP sequence | this study |
pLTEX-4 | based on pLTEX-1; changed SP sequence | this study |
pLTEX-5 | based on pLTEX-1; changed SP sequence | this study |
pLTEX-2_scFv-1 | Vector for expression-secretion of scFv-1 | this study |
pLTEX-3_scFv-1 | Vector for expression-secretion of scFv-1 | this study |
pLTEX-4_scFv-1 | Vector for expression-secretion of scFv-1 | this study |
pLTEX-5_scFv-1 | Vector for expression-secretion of scFv-1 | this study |
pLTEX-2_scFv-2 | Vector for expression-secretion of scFv-2 | this study |
pLTEX-3_scFv-2 | Vector for expression-secretion of scFv-2 | this study |
pLTEX-4_scFv-2 | Vector for expression-secretion of scFv-2 | this study |
pLTEX-5_scFv-2 | Vector for expression-secretion of scFv-2 | this study |
pLTEX-2_scFv-3 | Vector for expression-secretion of scFv-3 | this study |
pLTEX-3_scFv-3 | Vector for expression-secretion of scFv-3 | this study |
pLTEX-4_scFv-3 | Vector for expression-secretion of scFv-3 | this study |
pLTEX-5_scFv-3 | Vector for expression-secretion of scFv-3 | this study |
pLTEX-2_scFv-4 | Vector for expression-secretion of scFv-4 | this study |
pLTEX-3_scFv-4 | Vector for expression-secretion of scFv-4 | this study |
pLTEX-4_scFv-4 | Vector for expression-secretion of scFv-4 | this study |
pLTEX-5_scFv-4 | Vector for expression-secretion of scFv-4 | this study |