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Figure 1 | Microbial Cell Factories

Figure 1

From: Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae

Figure 1

SignalP 3.0 analysis of different SP-sequences. Graphical output of SignalP-HMM, showing the posterior probabilities for n-, h- and c-region and cleavage sites. (A) human IgG VH leader peptide with scFv for expression in human cells (B) Erwinia carotovora pelB SP with human scFv for E. coli expression; (C) Leishmania mexicana secreted acid phosphatase 1 (SAP1); (D) the SAP1 SP-sequence with human scFv neglecting any cloning strategy; (E). the SAP1 SP-sequence in pLEXSY-sat2 with scFv cloned via available XbaI RE-site.

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Microbial Cell Factories

ISSN: 1475-2859

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