From: Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production
Method | Procedure |
---|---|
Molecular distillation (Fractional distillation) | Purification of fatty acid esters in a vacuum system based on the different boiling points of different fatty acids [105]. |
Molecular sieves | Separation via membrane permeability and selectivity [106]. |
PUFA transformations | Esterification of PUFA and free fatty acids to produce esters (ethyl-, glyceryl-, sugar-, other). Inter-esterification to enrich lowly unsaturated fatty acids with PUFA [107]. |
Super Critical Fluid Extraction | Optimization of lipid solubility and fractionation in supercritical CO2[108]. |
Urea complexation | Solubilization of fatty acids, adding urea and ethanol to saturation point exposing it to heat. Recovery of product by filtration [109]. |
Winterization | Temperature reduction to render more saturated fats insoluble [110]. |