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Table 2 Primers used for S. cerevisiae chromosomal integrations

From: Physiological heterogeneities in microbial populations and implications for physical stress tolerance

Primer

Sequence 5'-3'

FP169, r

TATAAAGGCCATGAAGCTTTTTCTTTCCAATTT TTTTTTTTTCGTCATTA

 

TAGAAATCATTACGACCGAGATTCCCGGGTAA TTGGCCGCAAATTAAAGC

FP212, f

GATTCGGTAATCTCCGAGCAGAAGGAAGAACGA AGGAAGGAGCAC

 

CAGCTATGACCATGATTACG

FP213, r

TTTTTCGTCATTATAGAAATCATTACGACCGAG ATTCCCGGGTAA

 

TTTTTGATCGGGTAATAACTG

  1. The underlined sequence is complementary to the PCR template; the remaining part of the sequence is complementary to the ura3-52 chromosomal site in CEN.PK113-5D.
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Microbial Cell Factories

ISSN: 1475-2859

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