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Table 2 Specific product yield (AU/gm DCW) of various clones under three different conditions, uninduced, induced with arabinose and induced with arabinose and IPTG both (6 hrs post induction)

From: An inverse metabolic engineering approach for the design of an improved host platform for over-expression of recombinant proteins in Escherichia coli

a) Clones in pRSET A

Clone name

Specific product yield (Ara + IPTG)

Specific product yield (Ara)

Specific product yield (Uninduced)

EN34 (mfd)

362

91

14

G18 (galT)

76

227

18

GN35

89

244

9

GN11 (ribB)

347

47

8

MN45 (aroC)

67

199

25

BN43 (kdpD)

256

67

10

b) Clones in pBAD 33

   

A17 (bioF)

125

181

9

A29 (kdpF)

681

79

5

F50

225

207

10

L27

175

175

13