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Table 1 Comparison of calculated and mass spectrometry determined molecular masses of fusion protein and peptide samples

From: Recombinant production of self-assembling β-structured peptides using SUMO as a fusion partner

Protein/peptide sample

Calculated mass (Da)‡

Observed mass (Da)

Difference (Da)

SUMO_P11-4

14821.4

14820.8

−0.6

SUMO_P11-13

14796.3

14796.4

+0.1

SUMO_P11-14 (K)

14790.5

14789.6

−0.9

SUMO cleaved

13284.8

13284.0

−0.8

P11-4

1554.6

1553.7

−0.9

P11-13

1529.5

1528.6

−0.9

P11-14 (K)

1523.8

1522.8

−1.0

  1. ‡ The calculated molecular mass is of SUMO protein lacking the N-terminal methionine residue.