Figure 2
From: Recombinant production of self-assembling β-structured peptides using SUMO as a fusion partner
SDS-PAGE gels showing the cleavage of SUMO_P 11 -N with SUMO protease. A) Uncleaved and SUMO protease cleaved SUMO_P11-4 in either buffer (lanes 1 and 2) or water (lanes 3 and 4). B) SUMO_P11-13 uncleaved (lane 1) and SUMO protease cleaved in water (lane 2) and C) SUMO_P11-14 (K) uncleaved (lane 1) and SUMO protease cleaved in water (lane 2). Lanes 3 and 4 show overloaded samples of Lanes 1 and 2 respectively to allow visualisation the released P11-14 (K) peptide (lane 4). Lane 5 shows SUMO protease.