Table 1 Strain and plasmids used in this work
From: Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity
Name | Genotype | Reference |
|---|---|---|
BW25113 | rrnBT14 ΔlacZ WJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | [27] |
JCL16 | BW25113/F’ [traD36, proAB+, lacIq ZΔM15] | [17] |
JCL260 | JCL16: ΔadhE; ΔfrdBC; Δpta; Δfnr-ldhA; ΔpflB | [5] |
SA542 | JCL260: ΔyqhD | [16] |
AL287 | SA542: ΔadhP | This work |
AL288 | SA542: ΔeutG | This work |
AL289 | SA542: ΔyiaY | This work |
AL290 | SA542: ΔyjgB | This work |
AL555 | SA542: ΔbetA | This work |
AL615 | SA542: ΔfucO | This work |
AL616 | SA542: ΔeutE | This work |
AL312 | SA542: ΔadhP ΔeutG | This work |
AL322 | SA542: ΔadhP ΔeutG ΔyiaY | This work |
AL329 | SA542: ΔadhP ΔeutG ΔyiaY ΔyjgB | This work |
AL556 | SA542: ΔadhP ΔeutG ΔyiaY ΔyjgB ΔbetA | This work |
AL626 | SA542:ΔadhP ΔeutG ΔyiaY ΔyjgB ΔbetA ΔfucO | This work |
AL707 | SA542:ΔadhP ΔeutG ΔyiaY ΔyjgB ΔbetA ΔfucO ΔeutE | This work |
Plasmids | Features | Reference |
pSA69 | p15A ori; KanR; PLlacO1: alsS-ilvCD | [5] |
pSA129 | ColE1 ori; AmpR; PLlacO1: kivd | [16] |
pSA138 | ColE1 ori; PLlacO1: kivd-yqhD | [5] |
pGR03 | p15A ori; CmR; PLlacO1: alsS-ilvCD | This work |
pAL217 | ColE1 ori; AmpR; PLlacO1: kivd-adhP | This work |
pAL218 | ColE1 ori; AmpR; PLlacO1: kivd-eutG | This work |
pAL219 | ColE1 ori; AmpR; PLlacO1: kivd-yiaY | This work |
pAL220 | ColE1 ori; AmpR; PLlacO1: kivd-yjgB | This work |
pAL221 | ColE1 ori; AmpR; PLlacO1: kivd-betA | This work |
pAL222 | ColE1 ori; AmpR; PLlacO1: kivd-fucO | This work |
pAL223 | ColE1 ori; AmpR; PLlacO1: kivd-eutE | This work |
pZE12-luc | ColE1 ori; AmpR; PLlacO1: luc(VF) | [28] |
pAL162 | ColE1 ori; AmpR; PLlacO1: adhP | This work |
pAL158 | ColE1 ori; AmpR; PLlacO1: eutG | This work |
pAL157 | ColE1 ori; AmpR; PLlacO1: yiaY | This work |
pAL156 | ColE1 ori; AmpR; PLlacO1: yjgB | This work |
pAL213 | ColE1 ori; AmpR; PLlacO1: betA | This work |
pAL214 | ColE1 ori; AmpR; PLlacO1: fucO | This work |
pAL215 | ColE1 ori; AmpR; PLlacO1: eutE | This work |