Figure 4

Aggregation kinetics of Sup35-NM and Ure2p. The aggregation reactions of 20 μM yeast prionogenic proteins were carried out under agitation at 37°C. 2 μM of in vitro formed fibrils (representing 10% of the final protein concentration) or IBs (at a final OD_350nm of 0.125) were used for seeding and cross-seeding assays. The fibrillar fraction of Sup35-NM (A) and Ure2p (B) is represented as a function of time. The formation of Sup35-NM and Ure2p amyloid fibrils are accelerated only in the presence of pre-aggregated homologous protein, either fibrils or IBs.