Microbial Cell Factories

Table 3 e-FSCs against perturbed pgi gene expression

From: Investigating the effects of perturbations to pgi and eno gene expression on central carbon metabolism in Escherichia coli using ¹³ C metabolic flux analysis

J_kfor $C_{pgi}^{J_{k}}$ ^a	IPTG concentration in culture ofpgiexpression-controllable strain (μg/ml)^b
	0 (0.23)	20 (1.25)	50 (2.80)	100 (4.16)
J₁–J₂	4.00	3.98	3.60	2.59
J₁₀–J₁₁	0.08	0.03	0.01	0.01
J₁₄	0.00	0.06	0.14	0.15
J₁₅	0.00	0.04	0.79	1.78
J₁₉–J₂₀	0.00	0.30	1.53	1.92
J₂₂	–0.01	–0.39	–4.77	–8.57
J₂₃	0.00	–0.06	–0.27	–0.55
J₂₄	0.01	0.00	0.00	0.00
J₃₃	–0.02	–0.28	–0.31	–0.21

^ae-FSC was estimated from metabolic flux distributions and pgi expression levels of the pgi expression-controllable-strain YUEC04 and pgi knockout strain JWK3985.
^bValue in parentheses represents the pgi expression level shown in Figure 2.

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ISSN: 1475-2859

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