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Figure 4 | Microbial Cell Factories

Figure 4

From: Investigating the effects of perturbations to pgi and eno gene expression on central carbon metabolism in Escherichia coli using 13 C metabolic flux analysis

Figure 4

Effect of perturbed pgi gene expression on metabolic flux in E. coli . All fluxes were normalized to the specific glucose uptake rate of 100 to allow comparison of the flux distributions at different glucose uptake rates. Metabolic flux distributions of the pgi-knockout strain JWK3985 (a), pgi expression-controllable strain YUEC04 in the presence of 0 μg/mL IPTG (b), 20 μg/mL IPTG (c), 50 μg/mL IPTG (d), and 100 μg/mL IPTG (e) and the control strain YUEC00 (f) are shown. As for the reversible reactions, net fluxes are shown. G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; GAP, glyceraldehyde-3-phosphate; PGA, 3-phosphoglycerate; PEP, phosphoenolpyruvate; Pyr, pyruvate; AcCoA, acetyl-CoA; IsoCit, isocitrate; Mal, malate; Oxa, oxaloacetate; 6PG, 6-phosphogluconate.

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Microbial Cell Factories

ISSN: 1475-2859

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