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Table 1 Primers used in this study

From: A structurally informed autotransporter platform for efficient heterologous protein secretion and display

Primer

Sequence (5’ → 3’)

Hbp(down XmaI) rv

gtaccgacaaattctccctg

Hbp BamHI QC fw

cttcatcactgaagttggttccctgaacaaacgcatg

Hbp BamHI QC rv

catgcgtttgttcagggaaccaacttcagtgatgaag

Hbp(Δd1) fw

gggagctcctgcggatccggcagcggtaatgatg ccccggtcacgttc

Hbp(Δd1) rv

cggatccgcaggagctccccgcaagacttcctgc agag

Hbp(Δd2) fw

ctgggagctccgcaggatccggcagcggtaatac tgcagggtatctgtttc

Hbp(Δd2) rv

ctgccggatcctgcggagctcccagaaccggcata gtccagcgtgatag

Hbp(Δd3) fw

gggagcgggagctccgcaggatccggcagcgg taaccgcagttttacctttgac

Hbp(Δd3) rv

accgctgccggatcctgcggagctcccgctcccct gcagcgtcagacg

Hbp(Δd4) fw

gggagcgggagctccgcaggatccggcagcggt agtgtcttcaacggcaccg

Hbp(Δd4) rv

accgctgccggatcctgcggagctcccgctcccgtc gcccagcgtgacgctg

Hbp(Δd5) fw

gggagcgggagctccgcaggatccggcagcggg taccgcaatatctggagc

Hbp(Δd5) rv

gctgccggatcctgcggagctcccgctccctccgag ggtgacagtc

Hbp(Tr557) fw

gcggggagctccgcaggatccggcagcggtgcag ggtatctgtttcacgg

Hbp(Tr804) fw

gcggggagctccgcaggatccggcagcggtttacc tgtatacgattatgccg

Hbp(Tr868) fw

gcggggagctccgcaggatccggcagcgggtacc gcaatatctggagcgg

Hbp(Tr942) fw

gggagctcctgcggatccggcagcggtgcagacaa actggtgataaac

Hbp EcoRI rv

cagtgaattctcagaatgaataacgaatattag

lacUV5_ScaI_fw

gcgcagtactttgcgccattctatggtgtc

pEH3Hbpβ_ScaI_rv

gcgcagtactcacagcatcagaatgaataacg

malE_insert_seq

tataacccttgtcgccgttg

malK_insert_seq

acgcagcaaggtcgatttac

pEH_XbaI_EspC_fw

taactttctagattacaaaacttaggagggtttttacca tgaataaaatatacgcattaaaata

EcoRI_EspC rv

gtcagaattctcagaaagaataacggaagttag

EspC(Δdom1/Cas) fw

gggagctccgcaggatccggcagcggtttaaaaaa caaatttactcaaaaagtc

EspC(Δdom1/Cas) rv

cggatcctgcggagctcccagcctgagatgcgctta aaaaag

EspC (BglII) rv

ccagagccaatgtttacgtc