Table 2 Sequences of primers used for construction of expression cassettes
From: Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters
Name | Sequence (5′–3′) | Amplification target |
|---|---|---|
Ppdc-F | cccaagctt aggacttccagggctacttg (Hind III) | pdc promoter |
Ppdc-R | gattgtgctgtagctgcgct | |
xyn-p-F | atggtctccttcacctccctc | xyn coding sequences |
xyn-p-R | gcaactgcag cacatcacaaaagaagagcccc (Pst I) | |
Tpdc-F | gcaactgcag cccggcatgaagtctgacc (Pst I) | pdc terminator |
Tpdc-R | gctctaga tggacgcctcgatgtcttcc (Xba I) | |
Peno-F | gcaactgcag tgattccgtcctggattgc (BamH I) | eno promoter |
Peno-R | tttgaagctatttcaggtggctgg | |
xyn-e-F | atggtctccttcacctccctc | xyn coding sequences |
xyn-e-R | ggggtacc cacatcacaaaagaagagcccc (Kpn I) | |
Teno-F | ggggtacc atggccacgagagacaactacc (Kpn I) | eno terminator |
Teno-R | ggaattc tggcgtcgttgatgtttcg (EcoR I) | |
Pgpd-F | gctaagctt gacgcagaagaaggaaatcgcc (Hind III) | gpd promoter |
Pgpd-R | tttgtatctgcgaattgagcttgcg | |
xyn-g-F | atggtctccttcacctccctc | xyn coding sequences |
xyn-g-R | gcaactgcag cacatcacaaaagaagagcccc (Pst I) | |
Tgpd-F | gcaactgcag gtgctgtgttcctcagaatggg (Pst I) | gpd terminator |
Tgpd-R | gctctaga ttacggatctgatcactcggg (Xba I) |