Table 1 Sequences of primers used in cDNA synthesis
From: Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters
Name | Sequence (5′–3′) | Amplification target |
|---|---|---|
sar-1 | tggatcgtcaactggttctacga | sar1 cDNA |
sar-2 | gcatgtgtagcaacgtggtcttt | |
gpd-F | gtgctgcccagaacatcatcc | gpd cDNA |
gpd-R | tggcggtagggacacgaatg | |
pdc-F | ttcaacactgcgggcttct | pdc cDNA |
pdc-R | cagaacacccctcatcgctac | |
kdh-F | aggaaaccgaggagacatatacc | kdh cDNA |
kdh-R | gctcaaggacgagttggaaatg | |
pda-F | aacctccctgctctgtttggc | pda cDNA |
pda-R | tcctcgcttgtagtagtcggtca | |
eno-F | agcttgccgccatgtactctg | eno cDNA |
eno-R | aatctggatgtcctgggtcttg | |
ald1-F | agaagacggagcagtagttgagg | ald1 cDNA |
ald1-R | tgagaagattgtgggttggatg | |
glk-F | aagccaacgcgggattaaag | glk cDNA |
glk-R | gtgccgcccaggtctacag | |
pyk-F | cctcagcatcaaggacgaccag | pyk cDNA |
pyk-R | acatcggtgttgggcaggttg | |
cit-F | ggacctcctccgtctctaccttg | cit cDNA |
cit-R | ggtcactcagggcactaccaaca | |
adh-F | cttccagcaggcaaccgagt | adh cDNA |
adh-R | tggtgatcatgcggatgacg | |
fba-F | cctgtgcggtatccgtgact | fba cDNA |
fba-R | ggacatggtcttctcaccctcg | |
tpi-F | aacgagaagaactgcggtgaac | tpi cDNA |
tpi-R | gacgatgtcaatgaaggcagg | |
ald2-F | gagggcaagacgctggaca | ald2 cDNA |
ald2-R | gcatgagcagggggaagttc |