Figure 4

(A) Gas-chromatography analysis patterns of monohydroxylated product. A major oxidative metabolite of daidzein was separated and identified as 3’,4’,7-trihydroxyisoflavone (3’-ODI) based on the retention times and mass (m/z) values comparing with reference compound. (B) Mass spectral analysis revealed that each had a molecular ion mass of 486 m/z after BSTFA derivatization, which was exactly the same as those of the reference compounds. As a result, 3’-ASDH oxidized daidzein to mono-hydroxylated product of daidzein with very high catalytic activity.