Figure 3

Expression and UV-visible absorption spectra of 3’-ASDH. (A) SDS/PAGE analysis of total cell protein content as well as purified protein containing the His-6 tag; the lanes, from the left, are total cell protein content after induction, total soluble protein content, purified protein content and total cell protein content before IPTG and protein marker, respectively. (B) UV-visible absorbance spectra of purified 3’-ASDH fusion protein revealed spectral features characteristic of CYPs; Dashed line: oxidized form, Dotted line: Reduced form with dithionite. Solid line: CO-difference spectra; Inserted figure: CO-difference spectra of 3’-ASDH.