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Figure 2 | Microbial Cell Factories

Figure 2

From: Engineering of daidzein 3’-hydroxylase P450 enzyme into catalytically self-sufficient cytochrome P450

Figure 2

Design of artificial self-sufficient daidzein hydroxylases by heme domain swapping with self-sufficient CYP102D1 from Streptomyces avermitilis . The reductase domain consisting of di-flavin binding domain (FAD/FMN) has linker sequences from Val458 and the coding synthetic DNA was first cloned into the expression vector pET24m(a). And the target DNAs coding daidzein hydroxylases were amplified with each specific primer and coned into the pET24m(a)-CYP102D1 reductase.

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