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Figure 6 | Microbial Cell Factories

Figure 6

From: Improved adhesive properties of recombinant bifidobacteria expressing the Bifidobacterium bifidum-specific lipoprotein BopA

Figure 6

Adhesion of bifidobacteria overexpressing bopA. His6-specific Western Blot on lysates of B. bifidum S17 (wt), B. bifidum S17 pMGS_Pgap_bopA His6 (A) or B. longum/infantis E18 (wt), and B. longum/infantis E18 pMGS_Pgap_bopA His6 (B) grown for 16 h. Lysates of E. coli DH10B pMGS_Pgap_bopA His6 were used as positive control for detection of BopA-His6. Adhesion of B. bifidum S17 pMGS_Pgap_bopA His6 (C) and B. longum/infantis E18 pMGS_Pgap_bopA His6 (D) to T84 (black bars), Caco-2 (light grey bars) and HT29 (dark grey bars) cells compared to their parental wildtype strains. Results are expressed as percent adherent bacteria relative to the initially added colony forming units. Values are mean ± standard deviation of one representative of at least three independent experiments performed in triplicate measurements. Statistical analysis was performed by pairwise comparison to the respective positive control (i.e. adhesion of B. bifidum S17 or B. longum/infantis E18 wildtype) using Students t-test. Letters above bars indicate statistical significance (a: p < 0.05; b: p < 0.01; c: p < 0.001).

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