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Figure 3 | Microbial Cell Factories

Figure 3

From: Improved adhesive properties of recombinant bifidobacteria expressing the Bifidobacterium bifidum-specific lipoprotein BopA

Figure 3

Detection of bopA in B. bifidum strains and expression analysis. (A) Southern Blot analysis for bopA homologues using chromosomal DNA of B. bifidum strains S16, S17, NCC189 and NCIMB41171, B. breve strains S27 and DSM20213, B. longum/infantis strains E18 and NCC200, B. adolescentis strains NCC251 and DSM20083, B. lactis strains NCC362 and L15, B. animalis MB254, and B. longum strains NCC2705 and DSM20219. As positive control for hybridisation, a non-labelled PCR product of bopA of B. bifidum S17 was used. (B) Expression analysis of bopA was performed by Northern Blot using RNA isolated from B. bifidum strains S16, S17, NCC189 and NCIMB41171 after growth for 16 h in MRSC medium. As negative control RNA isolated from B. longum/infantis E18 was used. (C) Schematic representation of the bopA locus with localisation of oligonucleotides used for amplification of the probe for Southern Blot (1,812 bp) and Northern Blot (489 bp).

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