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Table 3 Oligonucleotide primers for the construction process

From: Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Name Primers 5'→3'
cutinase-F GTAATCCATATG GCCAACCCCTACGAGCGC
cutinase-HlyAs-F CTCCACCTGCCCGTTCTTAGCCTATGGAAGTC
cutinase-HlyAs-R GACTTCCATAGGCTAAGAACGGGCAGGTGGAG
HlyAs-R CCGCTCGAG TTATGCTGATGCTGTCAAAG
HlyAs-MF GCCAGTGATTTTTCgTATGGACGGAACTC
HlyAs-MR GAGTTCCGTCGATAcGAAAAATCACTGGC
HlyBD-F CGGCGAGCTC GGATTCTTGTCATAAAATTG
HlyBD-R CCACGGATCC TTAACGCTCATGTAAAC