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Table 3 Oligonucleotide primers for the construction process

From: Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Name

Primers 5'→3'

cutinase-F

GTAATCCATATG GCCAACCCCTACGAGCGC

cutinase-HlyAs-F

CTCCACCTGCCCGTTCTTAGCCTATGGAAGTC

cutinase-HlyAs-R

GACTTCCATAGGCTAAGAACGGGCAGGTGGAG

HlyAs-R

CCGCTCGAG TTATGCTGATGCTGTCAAAG

HlyAs-MF

GCCAGTGATTTTTCgTATGGACGGAACTC

HlyAs-MR

GAGTTCCGTCGATAcGAAAAATCACTGGC

HlyBD-F

CGGCGAGCTC GGATTCTTGTCATAAAATTG

HlyBD-R

CCACGGATCC TTAACGCTCATGTAAAC