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Table 2 Monomeric products released from cutin hydrolysis by cutinase and cutinase-HlyAs, respectively

From: Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Cutin hydrolysis products Cutinase Hydrolysis
Area (%)
Cutinase-HlyAs Hydrolysis Area (%)
Hexadecanoic acid 34.17 31.78
Octadecanoic acid 33.63 35.57
9-Octadecenoic acid 1.16 1.18
9,12-Octadecadienoic acid 1.08 1.65
16-Hydroxy hexadecanoic acid 0.34 0.40
18-Hydroxyoctadeca-9,12-dienoic acid 1.46 1.55
  1. Cutin hydrolysis by cutinase-HlyAs and cutinase were incubated in 25 mM potassium phosphate buffer (pH 8.0) at temperature 50°C for 18 h. The results are the averages of triplicate assays.