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Table 2 Monomeric products released from cutin hydrolysis by cutinase and cutinase-HlyAs, respectively

From: Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Cutin hydrolysis products

Cutinase Hydrolysis

Area (%)

Cutinase-HlyAs Hydrolysis Area (%)

Hexadecanoic acid

34.17

31.78

Octadecanoic acid

33.63

35.57

9-Octadecenoic acid

1.16

1.18

9,12-Octadecadienoic acid

1.08

1.65

16-Hydroxy hexadecanoic acid

0.34

0.40

18-Hydroxyoctadeca-9,12-dienoic acid

1.46

1.55

  1. Cutin hydrolysis by cutinase-HlyAs and cutinase were incubated in 25 mM potassium phosphate buffer (pH 8.0) at temperature 50°C for 18 h. The results are the averages of triplicate assays.
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Microbial Cell Factories

ISSN: 1475-2859

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