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Table 1 Summary of the purification of cutinase-HlyAs

From: Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Purification step Total protein
(mg)
Total activity
(U)
Specific activity
(U/mg)
Yield
(%)
Purification
(fold)
Crude extract 584.9 128497.5 219.7 100 1
Ammonium sulfate fraction 481.7 113334.8 235.3 88.2 1.1
DEAE Sepharose purification 129.9 51369.9 395.5 40.0 1.8
MonoQ purification 68.8 30681.8 446.2 23.9 2.0