Figure 3From: Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)Effects of temperature on activity and stability of cutinase-HlyAs and cutinase. (A) Temperature optimum determined in Tris-HCl buffer (pH 8.0); (B) Thermostability of the enzymes in Tris-HCl (pH 8.0) at 50°C. ■, cutinase; ●, cutinase-HlyAs. The error bars show the standard deviations from three measurements.Back to article page