Figure 4

Overexpression of CsrB improves production through engineered biofuel pathways. A) Overexpression of CsrB improved production of total free fatty acids, detected as fatty acid methyl esters (FAMEs) by 1.8 fold in E. coli overexpressing L-tesA after 72 hrs relative to an empty plasmid control. Overexpressed E. coli genes are indicated in color. B) Inclusion of overexpressed CsrB nearly doubled total productionof 1-butanol yielded from E. coli co-expressing the C. acetobutylicum butanol pathway, relative to an empty plasmid control. C. acetobutylicumgenes are designated in bold font. C) CsrB overexpressed with an engineered mevalonate pathway and the amorphadiene synthase (ADS) geneproduced approximately twice the amorphadiene after 48 hrs relative to a control. Genes from S. cerevisiae and heterologous genes are designated in bold font.