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Table 2 GPCR variants constructed and evaluated in S. cerevisiae in this study

From: Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

GPCR

Variant name

Deletions and mutations

hADRB2

FL

full-length ADRB2 (1–413)

 

ADRB2mut

ADRB2(1–365), E122W, N187E

hCHRM2

FL

full-length CHRM2 (1–466)

 

Nd-i3d

CHRM2 (11–466), i3d(233–380)

 

Nd-M112W-i3d

CHRM2 (11–466), M112W, i3d(233–380)

hHRH1

FL

full-length HRH1 (1–487)

 

Nd-i3d

HRH1 (20–487), i3d (229–397)

 

Nd-T4L

HRH1 (20–487), T4L

 

Nd-F116W-i3d

HRH1 (20–487), F116W, i3d (229–397)

 

Nd-F116W-T4L

HRH1 (20–487), F116W, T4L

hNTSR1

FL

full-length NTSR1 (1–418)

 

Nd-Cd

NTSR1 (43–385)

 

Nd-T4L-Cd

NTSR1 (43–385), T4L

 

Nd-L157W-Cd

NTSR1 (43–385), L157W

 

Nd-L157W-T4L-Cd

NTSR1 (43–385), L157W, T4L

  1. NTSR1 (43–385) means that the N- and C-terminal residues are truncated, the receptor starts from residue 43, and the receptor ends with residue 385. E122W, M112W, F116W and L157W are the mutations to tryptophan at position 3.41. Deletion of the i3-loop from position 229 to 297 is indicated as i3d (229–297). T4L indicates the fusion of the T4 lysozyme sequence between TM5 and TM6.
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Microbial Cell Factories

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