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Figure 4 | Microbial Cell Factories

Figure 4

From: Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

Figure 4

Purification of the receptor variant. (A) hHRH1-Nd-i3d expressed in P. pastoris, and (B) hHRH1-Nd-T4L expressed in S. cerevisiae. The size-exclusion chromatogram using Superdex 200 10/300 is shown in the left panel, and the Coomassie blue-stained SDS-PAGE gel is shown in the right panel. Lane 1, molecular weight marker. Lane 2, purified receptor variant. Arrows indicate the purified receptors.

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