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Figure 3 | Microbial Cell Factories

Figure 3

From: Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

Figure 3

Evaluation of the GPCR variants expressed in P. pastoris. The specific binding activities (left) and FSEC profiles (right) of full-length GPCRs and the improved GPCR variants expressed in P. pastoris are shown. The colors of the chromatogram correspond to those in the binding assays. (A) hADRB2, (B) hCHRM2, (C) hHRH1, (D) hNTSR1. FSEC was performed with a Superose 6 10/300 column. The void peak is denoted by an asterisk. The arrow indicates the target peak of GPCR fused to GFP.

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Microbial Cell Factories

ISSN: 1475-2859

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