Figure 2

Specific binding activity (left) and FSEC profile (right) of the full-length and improved variants expressed in S. cerevisiae. (A) hADRB2, (B) hCHRM2, (C) hHRH1, and (D) hNTSR1. FSEC was performed with the Superose 6 10/300 column. The colors of the FSEC chromatogram correspond to those of the binding activity. The void peak is denoted by an asterisk. An arrow indicates the target peak of GPCR-GFP fusion.