Table 1 Bacterial strains and plasmids used in the current study

E. coli DH5α

supE 44Δlac U169(Φ80 lacZ ΔM15) hdsR 17 recA 1 endA 1 gyrA 96 thi- 1 relA 1

Laboratory stock

P. putida

CCTCC AB92019

Cb^s, wild-type strain with pronounced vitality in wild environments

CCTCC^a stock

MB284

P. putida CCTCC AB92019 construct harboring pMB281

This study

MB285

P. putida CCTCC AB92019 construct harboring pMB282

This study

MB286

P. putida CCTCC AB92019 construct harboring pMB283

This study

Plasmids

pMB104

Amp^rCb^r, E. coli–P. putida shuttle vector containing P _oprL promoter and inaQ-N/gfp fusion gene, 6142 bp

[35]

pMB111

Amp^rCb^r, E. coli–P. putida shuttle vector containing P _oprL promoter and (inaQ-N)₂/gfp fusion gene, 6670 bp

Laboratory stock (unpublished)

pMB112

Amp^rCb^r, E. coli–P. putida shuttle vector containing P _oprL promoter and (inaQ-N)₃/gfp fusion gene, 7198 bp

Laboratory stock (unpublished)

pMB172

Amp^r; the recombinant plasmid carrying the mutated wlacD gene; 8,867 bp

[31]

pMB281

Amp^rCb^r, E. coli–P. putida shuttle vector containing P _oprL promoter and inaQ-N/wlacD fusion gene, 6872 bp

This study

pMB282

Amp^rCb^r, E. coli–P. putida shuttle vector containing P _oprL promoter and (inaQ-N)₂/wlacD fusion gene, 7404 bp

This study

pMB283

Amp^rCb^r, E. coli–P. putida shuttle vector containing P _oprL promoter and (inaQ-N)₃/wlacD fusion gene, 7926 bp

This study