Figure 3From: Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccaseMicrographs (a) and flow cytometric analysis (b) of recombinant P. putida strains. The cells were treated with anti-WlacD polyclonal antiserum followed by secondary Cy3-conjugated goat anti-mouse IgG for immunofluorescence microscopic examination or with goat anti-mouse Cy5-conjugate antibody for flow cytometric analysis. Panel (b): (i) P. putida AB92019 (negative control); (ii) P. putida MB284; (iii) P. putida MB285; and (iv) P. putida MB286. A total of 100,000 cells were analyzed for each flow cytometry experiment.Back to article page