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Table 1 Effect of pH up-shift on the secretion stress response in B. subtilis

From: High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by Bacillus subtilis

Strain Genotype Plasmid Na2CO3a LacZ activityb
R-1645 168 ΔamyE::P htrB -lacZ pHY300PLK - 6.7 ± 0.3
R-1645 168 ΔamyE::P htrB -lacZ pHY300PLK + 6.5 ± 0.5
R-1646 MGB874 ΔamyE::P htrB -lacZ pHY300PLK - 9.5 ± 0.1
R-1646 MGB874 ΔamyE::P htrB -lacZ pHY300PLK + 9.1 ± 0.3
R-1645 168 ΔamyE::P htrB -lacZ pHYK38 - 700 ± 86
R-1645 168 ΔamyE::P htrB -lacZ pHYK38 + 200 ± 48
R-1646 MGB874 ΔamyE::P htrB -lacZ pHYK38 - 1700 ± 36
R-1646 MGB874 ΔamyE::P htrB -lacZ pHYK38 + 590 ± 85
  1. aB. subtilis derivative strains were grown in 2xL-Mal medium at 30°C. At the transition phase (12 h), a Na2CO3 solution was added to the growth medium at a final concentration of 0.9% (w/v). -, No addition; +, Addition.
  2. bLacZ activity was determined after 48 h of cultivation. β-galactosidase-specific activities are reported in units (U) calculated using the following formula: 1000 x A420/reaction time (min) x OD600 of culture.