Figure 3
Activity of native and recombinant enzymes before and after the deglycosylation treatment with Endo H. Different substrates were assayed for the analysis: p NPB (white bars), triolein-TO (gray bars), and cholesteryl oleate-CO (black bars). Substrates were used at 5 mM concentration in the presence of Genapol X-100. Conditions are described in experimental section. Data points are the average of at least two different measures. Error bars represent standard deviation.