Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans

Selvin, Joseph; Kennedy, Jonathan; Lejon, David PH; Kiran, G Seghal; Dobson, Alan DW

doi:10.1186/1475-2859-11-72

Table 1 Effects of physiochemical parameters on Lpc53E1 activity: substrate specificity, temperature, pH, metals, NaCl concentrations, detergents & others

From: Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans

Variables	Relative activity (%) (SD(yEr-))	Variables	Relative activity (%) (SD(yEr-))	Variables	Relative activity (%) (SD(yEr-))	Variables	Relative activity (%) (SD(yEr-))
Substrate specificity		Metals 1mM		Metals 10 mM		NaCl
p NPP	100.0 ± 0.7	Control	100.0 ± 0.1	Control	100.0 ± 0.1	0M	100 ± 0.08
p NPM	88.3± 2.4	Ag⁺	132.3 ± 3.5	Ag⁺	38.2 ± 2.5	1M	157.5 ± 4.7
p NPL	79.1 ± 9.1	Cu²⁺	135.1 ± 1.4	Cu²⁺	76.4 ± 5.8	1.5M	119.6 ± 5.4
p NPD	56.6± 3.3	Cr²⁺	147.4 ± 5.4	Cr²⁺	65.4 ± 5.5	2 M	157.5 ± 4.7
p NPC	32.1 ± 0.6	Mg²⁺	59.5 ± 0.1	Mg²⁺	17.1 ± 0.7	2.5M	164.7 ± 3.1
p NPB	28.4± 3.7	Mn²⁺	163.5 ± 5.0	Mn²⁺	42.4 ± 5.3	3M	170.3 ± 2.6
p NPA	23.5 ± 4.3	K⁺	106.4 ± 5.6	K⁺	30.5 ± 6.5	3.5M	164.6 ± 5.3
Temperature		Ca⁺	172.4 ± 0.5	Ca⁺	123.2 ± 0.3	4M	217.1 ± 0.8
4^oC	57.6 ± 5.1	Ni²⁺	98.1 ± 1.8	Ni²⁺	55.6 ± 5.7	4.5 M	226.2 ± 0.1
10^oC	57.7 ± 2.1	Fe³⁺	74.1 ± 1.2	Fe³⁺	72.1 ± 0.6	5M	234.3 ± 2.1
20^oC	63.5 ± 9.9	Pb²⁺	76.2 ± 1.9	Pb²⁺	58.1 ± 0.6	Detergents & others
30^oC	72.4 ± 3.6	Cd²⁺	67.2 ± 1.3	Cd²⁺	53.7 ± 4.6	EDTA (0.1%)	86.6 ± 4.9
40^oC	100.0 ± 1.2	Hg²⁺	62.3 ± 2.5	Hg²⁺	49.3 ± 1.5	Triton X (0.1%)	69.2 ± 1.8
50^oC	92.7 ± 2.9	Ba²⁺	29.1 ± 0.1	Ba²⁺	09.7 ± 5.0	Tween 80 (0.1%)	121.5 ± 4.7
60^oC	89.3 ± 2.7	Zn²⁺	88.1 ± 1.2	Zn²⁺	90.2 ± 3.4	SDS (0.1%)	32.2 ± 1.9
pH		Sn²⁺	34.1 ± 1.1	Sn²⁺	56.3 ± 5.2	CTAB (0.1%)	216.4 ± 3.4
pH3	62.3 ± 5.1	Li⁺	92.1 ± 1.1	Li⁺	78.0 ± 0.6	Gum arabic (0.1%)	75.6 ± 5.2
pH4	62.0 ± 0.6	Rb⁺	36.2 ± 1.7	Rb⁺	23.4 ± 1.0	DTT (0.1mM)	32.1 ± 0.6
pH5	54.7 ± 4.6	Cs⁺	78.1 ± 0.6	Cs⁺	36.0 ± 0.2	PMSF (0.1mM)	28.3 ± 2.4
pH6	68.3 ± 5.7	Al³⁺	53.1 ± 1.2	Al³⁺	32.3 ± 5.7	EDTA (1%)	33.2 ± 1.9
pH7	100.0 ±1.5	Co²⁺	56.5 ± 4.0	Co²⁺	28.3 ± 5.7	Triton X (1%)	16.4 ± 3.6
pH8	57.1 ± 1.1	CH₃COO^-	74.4 ± 5.6	CH₃COO^-	57.1 ± 1.4	Tween 80 (1%)	48.3 ± 2.5
pH9	73.3 ± 5.7	NO₃^-	82.1 ± 0.7	NO₃^-	64.1 ± 0.8	SDS (1%)	21.2 ± 1.8
pH10	84.3 ± 5.7	Cl^-	73.1 ± 0.2	Cl^-	89.2 ± 2.3	CTAB (1%)	102.2 ± 1.9
pH11	52.7 ± 1.5	SO₃^2-	83.5 ± 1.2	SO₃^2-	72.8 ± 5.4	Gum arabic (1%)	18.1 ± 1.2
pH12	50.6 ± 5.3	PO₄^3-	78.1 ± 4.3	PO₄^3-	85.3 ± 9.2	DTT (1mM)	11.4 ± 3.8
						PMSF (1mM)	4.6 ± 5.0

Mean relative activity values are shown with p- nitrophenyl palmitate as substrate. Values represent the mean of at least 3 separate determinations.

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ISSN: 1475-2859

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