Figure 4

3-D contour plots of relative enzyme activity with four variables (A: pNPP (mM), B: Ca²⁺(mM), C: Reaction time and D: NaCl). a). Ca²⁺(mM) and p NPP (mM), b). Reaction time (min) and p NPP (mM), c). NaCl (M) and Ca²⁺(mM), d). Reaction time (min) and Ca²⁺ (mM), e). NaCl and p NPP (mM) and f). NaCl (M) and Reaction time (min). The optimum Lpc53E1 activity of 2700U/mg at 1 mM pNPP, 5 mM Ca²⁺, 5 M NaCl at reaction time of 15 min, at pH 7 and temperature of 40°C predicted from the surface graphs.