Single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel

Table 3 Biodiesel properties of the transesterified SCOs of oleaginous fungi from mangrove wetlands

Property	Isolates					US biodiesel standard ASTM D6751	EU biodiesel standard EN 14214	Indian biodiesel standard IS 15607
Property	IBB B2	IBB F14	IBB G4	IBB G5	IBB M1	US biodiesel standard ASTM D6751	EU biodiesel standard EN 14214	Indian biodiesel standard IS 15607
Density ^* (g.cm^-3)	0.825 ^a	0.870 ^b	0.860 ^b	0.810 ^a	0.875 ^b	NS	0.86 – 0.90	0.86 – 0.90
Density ^* (g.cm^-3)	(0.857)	(0.865)	(0.865)	(0.855)	(0.865)	NS	0.86 – 0.90	0.86 – 0.90
Kinematic viscosity^# (40 °C; mm²s^-1)	4.52	4.69	4.55	4.55	4.68	1.9 – 6.0	3.5 – 5.0	3.5 – 5.0
SN ^*	195 ^a	217 ^b	201 ^a	190 ^a	200 ^a	NS	NS	NS
SN ^*	(193.2)	(193.2)	(195.3)	(193.0)	(192.1)	NS	NS	NS
IV ^*	91.5 ^a	60.28 ^b	73.0 ^c	76.30 ^c	54.81 ^b	NS	120max	NS
IV ^*	(74.9)	(53.4)	(52.5)	(69.7)	(67)	NS	120max	NS
HHV (MJ kg ^-1)^**	40.06	39.63	40.09	40.49	40.40	NS	NS	NS
HHV (MJ kg ^-1)^**	(40.38)	(40.7)	(40.6)	(40.47)	(40.55)	NS	NS	NS
CN ^#	56.22	61.24	61.2	57.815	58.42	47-65	51 min	51 min
TAN (mg KOH/g) ^*	ND	ND	0.2 ^a	0.1 ^b	0.1 ^b	0.8 max	0.5 max	0.5 max
FFA (%)^*	ND	ND	0.1 ^a	0.05 ^a	0.05 ^a	NS	NS	NS
Concentration of linolenic acid (C18:3) (%) ^*	0.5 ^a	ND	0.1 ^b	0.6 ^a	0.4 ^ab	NS	12 max	NS
FAME having ≥4 double bonds (%)^*	ND	ND	ND	ND	ND	NS	1 max	NS

The experimental values are means of three independent determinations. ^a,b,c Mean values within a row with different superscript letters differ significantly and were determined as mentioned in Methods (ANOVA, Tukey, p < 0.05). Separate analysis was done for each row.
ND not detected; NS not specified.
^* Experimentally determined values followed by predicted ones, if any, in brackets.
^# Predicted values as mentioned in Methods.
^** Calculated using experimental SN and IV while the values in brackets determined from predicted ones.

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